Sequence Alignment
The chart below shows the sequence alignment for the epitopes across several species.
Antibodies 1D9 and 5E2 cross react with mouse CFTR
Text in bold indicates 100% sequence identity to human CFTR. Mismatches are highlighted in bold magenta.
| Species | 1D9 | TJA9 | 154 | 660 | 8H16 | 1I22 | 450 | 570 | 432 | 217 | 596 | 5E2 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 528 | |||||||||||||||
| 769 | |||||||||||||||
| Human | ADNLSEKLEREWDRE | GRIIASYDPDNKEER | DSLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAP | KRKNS-ILNPI | PLERRLSLVPD-S | QARRRQSVL | IYSRRLSQETGLE | WPSGGQMT | EPSAHLDPVTYQIIR | |||
| Mouse | ADHLSEKLEREWDRE | GRIIASYDPENKVER | NNVSFSHLCLV | GYLDVFTEEQ | DTFDQFTEER | FSVDDSSA | KRKNS-ILNSF | LQEKRLSLVPD-S | PGRRRQSVL | VYSRRLSQDSTLN | WPSGGEMV | EPSAHLDPITYQVIR | |||
| Pig | ADNLSEKLEREWDRE | GRIIASYDPDNKAER | NSLFFSNFSLL | GYLDVLTEKE | DTFDQFTAER | FSLEGDAS | KRKNS-ILNSI | PLERRLSLVPD-S | QARRRQSVL | IYSRRLSQDTGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Rat | ADHLSEKLEREWDRE | GRIIASYDPDNTEER | NHLSFSHLCLV | GYLDVLTEEQ | DTFDQFTEER | FSVDD-AS | KRKNS-ILSSF | LQERRLSLVPD-S | PGRRRQSVL | IYSRRLSQDSTLN | WPSGGEMV | EPSANLDPITYQVIR | |||
| Sheep | ADNLSEKLEREWDRE | GRIIASYDPDNKVER | TSLFFSNL-LL | GYLDVLTEKE | DTFDQFTAER | FSLEGDTS | KRKNS-ILNSI | PLERRLSLVPH-S | LGGRRQSVL | IYSRRLSQDTGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Dog | ADHLSEKLEREWDRE | GRIIASYDPDNKQER | NSLFFSNFSLL | GYLDVLTEKE | DSFDQFSPER | FSLEGDAA | KRKNS-ILNPI | PVERRLSLFPD-C | RRQRRQSVL | IYSRRLSQDSGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Ferret | ADNLSEKLEREWDRE | GRIIASYDPDNKEER | NSLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAP | KRKNS-ILNPV | PLERRLSLVPDGA | QRQRRQSVL | IYSRRLSQDSGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Rabbit | ADNLSEKLEREWDRE | GRIIASYDPDNKVER | NNLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAS | KRKNS-ILNPV | SIERRLSLVPD-S | QGCRRQSVL | IYSRRLSQESGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Frog | ADNLSERLEREWDRE | GRIIASYDRDNEHER | PSAFFSNFSLH | SYLDLFTEKE | --FDHFNAER | CSIDSDPS | KRKSS-IINPR | QGERKLSLVPE-S | QGRRRQSVL | IYNRRLSQDSILE | WPSGGQMT | EPSAHLDPITFQIIR | |||
| Chicken | GRIIASYDPDNSSER | NNLFFSNFPLH | GYLDIFTEKE | DSFDQFSAER | FSFEGESM | KRKNSIIINPL | SPERRISLVPD-L | QSRRRQSVL | IYTRRLSRDSILD | WPSGGQMT | EPSAHLDPITSQVIR | ||||
| Shark | ADELSEMLEREWDRE | GRIIASYNAKNTYER | DGLFFSNFSLH | SHLDVTTEKD | VHFDSFSAER | CSVSSGDG | KRKSSLIVNPI | PGERHFSLIPE-N | QAHRRQSVL | IYSRRLSEDGSFE | WPSGGQMM | EPTAHLDPVTFQIIR | |||
| Killifish | ADTLSERLEREWDKE | GRIIASFDPFHAAER | DGLFFTNL--Y | THLDIATEKE | GSYDNISAER | VSVDETAG | KRKPSLILNPL | LSDRRFSVVPE-D | NG-RRQSVL | IYARRLSKDSVFD | WPNRGQLE | EPSSYLDPITLQVLR | |||
| Atlantic salmon | ADNLSERLEREWDRE | GRIIGSFDPFHAPER | AGLFFTNLY-- | THLDIVTEKE | EAYDNIKAER | VSIDESTV | KRKQSIILNPL | LSDRKFSVVPE-D | LSGQRRQSVL | IYARPLSKDSVYD | WPNQGHMD | EPSAFLDPITLQVLR | |||