Sequence Alignment
The chart below shows the sequence alignment for the epitopes across several species. RED text indicates match with human (click to enlarge).
Antibodies 1D9 and 5E2 cross react with mouse CFTR
Text in bold indicates 100% sequence identity to human CFTR. Mismatches are highlighted in bold magenta.
| Species | 1D9 | TJA9 | 154 | 660 | 8H16 | 1I22 | 450 | 570 | 432 | 217 | 596 | 5E2 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 528 | |||||||||||||||
| 769 | |||||||||||||||
| Human | ADNLSEKLEREWDRE | GRIIASYDPDNKEER | DSLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAP | KRKNS-ILNPI | PLERRLSLVPD-S | QARRRQSVL | IYSRRLSQETGLE | WPSGGQMT | EPSAHLDPVTYQIIR | |||
| Mouse | ADHLSEKLEREWDRE | GRIIASYDPENKVER | NNVSFSHLCLV | GYLDVFTEEQ | DTFDQFTEER | FSVDDSSA | KRKNS-ILNSF | LQEKRLSLVPD-S | PGRRRQSVL | VYSRRLSQDSTLN | WPSGGEMV | EPSAHLDPITYQVIR | |||
| Pig | ADNLSEKLEREWDRE | GRIIASYDPDNKAER | NSLFFSNFSLL | GYLDVLTEKE | DTFDQFTAER | FSLEGDAS | KRKNS-ILNSI | PLERRLSLVPD-S | QARRRQSVL | IYSRRLSQDTGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Rat | ADHLSEKLEREWDRE | GRIIASYDPDNTEER | NHLSFSHLCLV | GYLDVLTEEQ | DTFDQFTEER | FSVDD-AS | KRKNS-ILSSF | LQERRLSLVPD-S | PGRRRQSVL | IYSRRLSQDSTLN | WPSGGEMV | EPSANLDPITYQVIR | |||
| Sheep | ADNLSEKLEREWDRE | GRIIASYDPDNKVER | TSLFFSNL-LL | GYLDVLTEKE | DTFDQFTAER | FSLEGDTS | KRKNS-ILNSI | PLERRLSLVPH-S | LGGRRQSVL | IYSRRLSQDTGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Dog | ADHLSEKLEREWDRE | GRIIASYDPDNKQER | NSLFFSNFSLL | GYLDVLTEKE | DSFDQFSPER | FSLEGDAA | KRKNS-ILNPI | PVERRLSLFPD-C | RRQRRQSVL | IYSRRLSQDSGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Ferret | ADNLSEKLEREWDRE | GRIIASYDPDNKEER | NSLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAP | KRKNS-ILNPV | PLERRLSLVPDGA | QRQRRQSVL | IYSRRLSQDSGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Rabbit | ADNLSEKLEREWDRE | GRIIASYDPDNKVER | NNLFFSNFSLL | GYLDVLTEKE | DSFDQFSAER | FSLEGDAS | KRKNS-ILNPV | SIERRLSLVPD-S | QGCRRQSVL | IYSRRLSQESGLE | WPSGGQMT | EPSAHLDPITYQIIR | |||
| Frog | ADNLSERLEREWDRE | GRIIASYDRDNEHER | PSAFFSNFSLH | SYLDLFTEKE | --FDHFNAER | CSIDSDPS | KRKSS-IINPR | QGERKLSLVPE-S | QGRRRQSVL | IYNRRLSQDSILE | WPSGGQMT | EPSAHLDPITFQIIR | |||
| Chicken | GRIIASYDPDNSSER | NNLFFSNFPLH | GYLDIFTEKE | DSFDQFSAER | FSFEGESM | KRKNSIIINPL | SPERRISLVPD-L | QSRRRQSVL | IYTRRLSRDSILD | WPSGGQMT | EPSAHLDPITSQVIR | ||||
| Shark | ADELSEMLEREWDRE | GRIIASYNAKNTYER | DGLFFSNFSLH | SHLDVTTEKD | VHFDSFSAER | CSVSSGDG | KRKSSLIVNPI | PGERHFSLIPE-N | QAHRRQSVL | IYSRRLSEDGSFE | WPSGGQMM | EPTAHLDPVTFQIIR | |||
| Killifish | ADTLSERLEREWDKE | GRIIASFDPFHAAER | DGLFFTNL--Y | THLDIATEKE | GSYDNISAER | VSVDETAG | KRKPSLILNPL | LSDRRFSVVPE-D | NG-RRQSVL | IYARRLSKDSVFD | WPNRGQLE | EPSSYLDPITLQVLR | |||
| Atlantic salmon | ADNLSERLEREWDRE | GRIIGSFDPFHAPER | AGLFFTNLY-- | THLDIVTEKE | EAYDNIKAER | VSIDESTV | KRKQSIILNPL | LSDRKFSVVPE-D | LSGQRRQSVL | IYARPLSKDSVYD | WPNQGHMD | EPSAFLDPITLQVLR | |||